Basic MCQ on Cell and Tissue Culture – Answer

A culture medium is nutrient material prepared in the laboratory for the growth of microorganisms. microorganisms that grow in size and number on a culture medium are referred to as a culture. Few question on basic cell and tissue culture 1. What microorganisms can use oxygen when it is present …

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A culture medium is nutrient material prepared in the laboratory for the growth of microorganisms. microorganisms that grow in size and number on a culture medium are referred to as a culture. Few question on basic cell and tissue culture

1. What microorganisms can use oxygen when it is present and continue to grow without it?
(a) Obligate aerobes
(b) Facultative anaerobes
(c) Obligate anaerobes
(d) Free radicals

2. What microorganisms require oxygen?
(a) Obligate aerobes
(b) Facultative anaerobes
(c) Obligate anaerobes
(d) Free radicals

3. What microorganisms do not use oxygen and can actually be harmed by it?
(a) Obligate aerobes
(b) Facultative anaerobes
(c) Obligate anaerobes
(d) Free radicals

4. What is not a toxic form of oxygen?
(a) Singlet oxygen
(b) Facultative oxygen
(c) Superoxide free radicals
(d) Hydroxyl radical

5. What is a sterile culture medium?
(a) A hydroxyl radical medium
(b) A medium containing no living organisms
(c) A chemically defined medium
(d) A moisture-filled medium

6. What must chemically defined media contain?
(a) Growth factors
(b) Complex media
(c) Peptone complex
(d) Protein hydrolysis

7. What is agar?
(a) A nutrient
(b) Vitamins
(c) A solidifying agent
(d) Broth

8. What is an enrichment culture?
(a) Something that provides growth for all microorganisms
(b) Something that inhibits growth for all microorganisms
(c) An infectious culture
(d) Something that provides growth for a certain microorganism but not for others

9. What is an inoculating loop?
(a) A tool used to streak a microorganism in a pure culture
(b) A tool used to place agar in a pure medium
(c) A tool used to count colonies of microorganisms
(d) A tool used to view colonies of microorganisms

10. How can preserved bacteria cultures can be revived?
(a) Oxygenation
(b) Hydration
(c) Hydration and liquid nutrient medium
(d) Oxygenation and liquid nutrient medium

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